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Determination of phosphatase activity in osteoblasts by IR and two-dimensional correlation IR spectroscopy

  • 【作者】Ren, Zhongyuan,Zhang, Liping,Wu, Yuqing,Mebarek, Saida,Buchet, René
  • 【作者单位】1 State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun, China 2 College of Biology and Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China 3 Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, No. 1666, Kalun Street, Changchun 130062, PR China 4 Université de Lyon, Villeurbanne F-69622, France 5 Université Lyon 1, Villeurbanne F-69622, France 6 INSA-Lyon, Villeurbanne F-69622, France 7 CPE Lyon, Villeurbanne F-69622, France 8 ICBMS CNRS UMR 5246, Villeurbanne F-69622, France
  • 【年份】2016
  • 【卷号】Vol.86
  • 【页码】206-211
  • 【ISSN】0924-2031
  • 【关键词】IR spectroscopy Two-dimensional correlation IR spectroscopy Enzymatic assay 
  • 【摘要】 Phosphatase activity in osteoblasts like Saos-2 cells was previously determined by measuring infrared spectra of substrates (pyrophosphate, 4-nitrophenyl phosphate, α-glucose-1-phosphate or β-Glycerophosphate), in the presence of Saos-2 cells under p...
  • 【文献类型】 期刊
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