【作者】YaNan Qin ,Zhen Fang ,FengGuang Pan ,YaYa Zhao ,HongShan Li ,Han Wu ,Xianmei Meng
【作者单位】1. Laboratory of Nutrition and Functional Foods, Jilin University, 5333 Xi’an Road, Changchun, 130062, People’s Republic of China 2. Jilin Business and Technology College, 1606 Hao’yue Road, Changchun, 130062, People’s Republic of China
【摘要】 The calcium-binding residues, Tyr302 and His235, and the sodium-binding residue, Asp194, on the activity of Bacillus licheniformis α-amylase were investigated using site-directed mutagenesis. Tyr302 and His235 were replaced by Asn and Asp, respective...